全文获取类型
收费全文 | 420136篇 |
免费 | 53242篇 |
国内免费 | 537篇 |
出版年
2018年 | 3778篇 |
2016年 | 4844篇 |
2015年 | 6948篇 |
2014年 | 7989篇 |
2013年 | 11495篇 |
2012年 | 12754篇 |
2011年 | 12992篇 |
2010年 | 8710篇 |
2009年 | 8111篇 |
2008年 | 11345篇 |
2007年 | 11708篇 |
2006年 | 10886篇 |
2005年 | 10488篇 |
2004年 | 10066篇 |
2003年 | 10019篇 |
2002年 | 9625篇 |
2001年 | 19803篇 |
2000年 | 19990篇 |
1999年 | 15751篇 |
1998年 | 5384篇 |
1997年 | 5939篇 |
1996年 | 5615篇 |
1995年 | 5380篇 |
1994年 | 5288篇 |
1993年 | 5403篇 |
1992年 | 13377篇 |
1991年 | 13209篇 |
1990年 | 12577篇 |
1989年 | 12377篇 |
1988年 | 11288篇 |
1987年 | 10932篇 |
1986年 | 10218篇 |
1985年 | 10165篇 |
1984年 | 8503篇 |
1983年 | 7336篇 |
1982年 | 5644篇 |
1981年 | 5028篇 |
1980年 | 4839篇 |
1979年 | 7992篇 |
1978年 | 6391篇 |
1977年 | 5819篇 |
1976年 | 5481篇 |
1975年 | 6024篇 |
1974年 | 6221篇 |
1973年 | 6145篇 |
1972年 | 5551篇 |
1971年 | 5105篇 |
1970年 | 4257篇 |
1969年 | 4102篇 |
1968年 | 3674篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
92.
93.
94.
Karyotyping and hypervariable genetic markers indicate extensive leucochimaerism between pairs of dizygotic twins in cattle, a result of placental vascular anastomosis. The extent of this chimaerism includes both kind and number of cells exchanged. All heterosexual twin pairs harboured two types of leucocytes, having either XX or XY chromosome pairs, and 30 of 31 pairs of twins shared identical DNA fingerprints. Although chromosome results from skin fibroblasts indicate that some chimaerism occurs in the skin, the low level allows for differentiation of genotypes between twins. The results warrant against the common practice of using blood samples for DNA typing if twinning is not properly documented. 相似文献
95.
D W Carr Z E Hausken I D Fraser R E Stofko-Hahn J D Scott 《The Journal of biological chemistry》1992,267(19):13376-13382
The type II cAMP-dependent protein kinase (PKA) is localized to specific subcellular environments through binding of the dimeric regulatory subunit (RII) to anchoring proteins. Subcellular localization is likely to influence which substrates are most accessible to the catalytic subunit upon activation. We have previously shown that the RII-binding domains of four anchoring proteins contain sequences which exhibit a high probability of amphipathic helix formation (Carr, D. W., Stofko-Hahn, R. E., Fraser, I. D. C., Bishop, S. M., Acott, T. E., Brennan, R. G., and Scott J. D. (1991) J. Biol. Chem. 266, 14188-14192). In the present study we describe the cloning of a cDNA which encodes a 1015-amino acid segment of Ht 31. A synthetic peptide (Asp-Leu-Ile-Glu-Glu-Ala-Ala-Ser-Arg-Ile-Val-Asp-Ala-Val-Ile-Glu-Gln-Val -Lys-Ala-Ala-Tyr) representing residues 493-515 encompasses the minimum region of Ht 31 required for RII binding and blocks anchoring protein interaction with RII as detected by band-shift analysis. Structural analysis by circular dichroism suggests that this peptide can adopt an alpha-helical conformation. Both Ht 31 (493-515) peptide and its parent protein bind RII alpha or the type II PKA holoenzyme with high affinity. Equilibrium dialysis was used to calculate dissociation constants of 4.0 and 3.8 nM for Ht 31 peptide interaction with RII alpha and the type II PKA, respectively. A survey of nine different bovine tissues was conducted to identify RII binding proteins. Several bands were detected in each tissues using a 32P-RII overlay method. Addition of 0.4 microM Ht 31 (493-515) peptide to the reaction mixture blocked all RII binding. These data suggest that all anchoring proteins bind RII alpha at the same site as the Ht 31 peptide. The nanomolar affinity constant and the different patterns of RII-anchoring proteins in each tissue suggest that the type II alpha PKA holoenzyme may be specifically targeted to different locations in each type of cell. 相似文献
96.
97.
98.
99.
100.